目的:比较两种工艺制备的参芪扶正注射液对荷S180小鼠细胞免疫功能的影响。方法:将42只荷瘤小鼠随机分成5组,分别为对照组、现工艺制备的参芪扶正注射液高剂量组(8 g.kg-1)和临床等效剂量组(4 g.kg-1)、原工艺制备的参芪扶正注射液高剂量组(8 g.kg-1)和临床等效剂量组(4 g.kg-1)。腹腔注射给药,1次/d,实验组给予不同剂量的相应参芪扶正注射液,对照组给予生理盐水。7 d后从小鼠眼眶后静脉丛取血进行全血细胞检测及T淋巴细胞亚群检测。结果:4个实验组白细胞和淋巴细胞总数皆高于对照组,其中现工艺4 g.kg-1组与对照组的差异有统计学意义(P<0.01);4 g.kg-1剂量组中,现工艺淋巴细胞总数显著高于原工艺(P<0.05);现工艺4 g.kg-1组的CD4+淋巴细胞比例显著高于对照组(P<0.05);同一剂量两种工艺制剂之间T淋巴细胞亚群比例无显著差异。结论:在临床等效剂量上,现工艺制备的参芪扶正注射液可提高荷S180小鼠白细胞、淋巴细胞及CD4+T细胞的水平,效果优于或等于原工艺。 OBJECTIVE: To compare the effects of Shenqi Fuzheng Injection prepared by two methods on cellular immune function of S180 mice. METHODS: Forty-two tumor-bearing mice were randomly divided into five groups: the control group, the high-dose group of Shenqi Fuzheng Injection (8 g.kg-1) and the clinical equivalent dose group (4 g.kg). -1) The high-dose group (8 g.kg-1) and the clinical equivalent dose group (4 g.kg-1) of Shenqi Fuzheng Injection prepared by the original process. Intraperitoneal injection, once / d, the experimental group was given different doses of the corresponding Shenqi Fuzheng injection, the control group was given saline. Seven days later, blood was collected from the retro-orbital venous plexus of the mouse for detection of whole blood cells and detection of T lymphocyte subsets. Results: The total number of white blood cells and lymphocytes in the four experimental groups was higher than that of the control group, among which the difference between the current process 4 g.kg-1 group and the control group was statistically significant (P<0.01); 4 g.kg-1 dose group The total number of lymphocytes in the current process was significantly higher than the original process (P<0.05); the proportion of CD4+ lymphocytes in the 4 g.kg-1 group was significantly higher than that in the control group (P<0.05); There was no significant difference in the proportion of inter-T lymphocyte subsets. Conclusion: In terms of clinical equivalent dose, Shenqi Fuzheng injection prepared by current technology can increase the levels of white blood cells, lymphocytes, and CD4+ T cells in S180 mice, which is better than or equal to the original process.