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目的 :了解SEN病毒H亚型中国株的流行病学特点及其进化地位。方法 :结合已知的基因序列设计引物 ,建立了半套式PCR检测方法 ,对 5 8份献血者血清样本和 2 5份non_A_E肝炎患者血清样本进行了SENV_H亚型的检测 ,并对部分阳性血清的PCR产物进行克隆测序。结果 :检测结果显示无偿献血者中SENV_H亚型感染率为 34% ,在non_A_E肝炎患者中SENV_H亚型感染率为 2 4 % ,两者间无显著差异 ;但在无偿献血者中的阳性检出率大大高于国外报道。测序结果经BLASTP软件在GenBank中检索表明 ,各测序株均与已知的SENV_H株序列有较高的同源性 ,并在此基础上用MegAlign软件进行进化树分析。结论 :建立的半套式PCR检测方法适用于SEN病毒H亚型检测 ;SENV_H亚型中国株之间以及与已发表的国外SENV_H株序列之间都有较大的变异。与无偿献血者相比 ,non_A_E肝炎患者血清SENV_H株无进化上的显著倾向性。
Objective: To understand the epidemiological characteristics and evolution status of SEN virus H subtype Chinese strain. Methods: A set of primers was designed based on the known gene sequence. A semi-nested PCR assay was developed to detect SENV_H subtype in sera of 58 donors and 25 non_A_E hepatitis patients. Some positive sera The PCR products were cloned and sequenced. Results: The results showed that the prevalence of SENV_H subtype in unpaid blood donors was 34%. The prevalence of SENV_H subtype was 24% in non_A_E hepatitis patients, but there was no significant difference between the two groups The rate is much higher than foreign reports. The sequencing results were searched in GenBank by BLASTP software. The results showed that all the sequences had high homology with the known sequence of SENV_H strain. On the basis of this, the phylogenetic tree was analyzed by MegAlign software. CONCLUSION: The established semi-nested PCR detection method is suitable for the detection of SENV H subtypes. There is a large variation between SENV_H subtype Chinese strains and the published foreign SENV_H strain sequences. Serum SENV_H strains of non-AE hepatitis patients showed no significant evolutionary predisposition compared to non-compensated donors.