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目的:研究哮宁口服液的平喘、止咳、化痰和抗病毒作用,为临床治疗小儿哮喘提供依据。方法:采用离体气管螺旋条法观察哮宁口服液对气管平滑肌的松弛作用;采用氨水致小鼠咳嗽观察哮宁口服液对2min内咳嗽次数的影响;采用小鼠呼吸道酚红分泌实验观察哮宁口服液祛痰作用;采用体外抗病毒实验观察哮宁口服液对腺病毒3型(Adv3)、7型(Adv7)和呼吸道合胞病毒(RSV)的致细胞病变作用的影响。结果:离体气管螺旋条实验显示哮宁口服液20mg生药/ml、10mg生药/ml能使正常和组胺致痉的豚鼠气管螺旋条松驰;40g生药/kg和20g生药/kg能减少氨水导致小鼠咳嗽次数;增加小鼠呼吸道的酚红排泌量;体外抗病毒试验显示50mg生药/ml对3种病毒有明显抑制作用。结论:哮宁口服液具有平喘,止咳,化痰和抗病毒的药理作用。
OBJECTIVE: To study the antiasthmatic, cough-reducing, phlegm-resolving and antiviral effects of Zuoning Oral Liquid and provide the basis for clinical treatment of pediatric asthma. Methods: The relaxation of bronchial smooth muscle was observed by the method of the in vitro tracheal spirochete; the effect of the oral administration of the Zuoning oral liquid on the number of coughs within 2min was observed by using cough of ammonia-induced mice; Ning oral solution expectorant effect; in vitro antiviral experiment was used to observe the effect of the Zuning oral liquid on the cytopathic effect of adenovirus type 3 (Adv3), type 7 (Adv7) and respiratory syncytial virus (RSV). Results: In vitro tracheal spirochetal test showed that 20mg crude drug / ml and 10mg crude drug / ml of Zuoning oral solution can relax the trachea spiral strips of normal and histamine spasm guinea pigs; 40g crude drug / kg and 20g crude drug / kg can reduce ammonia Resulting in the number of mice coughing; increase the respiratory phenoloxidase excretion in mice; in vitro antiviral tests showed that 50mg crude drug / ml on the three viruses were significantly inhibited. Conclusion: Zuning oral solution has antiasthmatic, cough, phlegm and anti-virus pharmacological effects.