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利用辐射与离体培养、细胞筛选相结合的方法进行抗病突变体的筛选,选育出4个抗赤霉病的突变体。经多年田间接种鉴定和考察,突变体的抗病能力较亲本提高1~2级,株高、穗长和小穗数等主要农艺性状也发生了变异。突变体与其亲本相比,醇溶蛋白电泳和过氧化物同工酶酶谱的谱带数、谱带强度和谱带位置都有差异。超氧化物歧化酶的活性分析表明,在毒素作用下,突变体的酶活性比亲本高8,61%~24.14%。突变体具有较高的超氧化物畦化酶活性,是其具有赤霉病抗性的一种病理学原因。
Screening of disease-resistant mutants by means of combination of radiation and in vitro culture and cell screening, and breeding four mutant strains resistant to Fusarium head blight. After many years of inoculation, the disease resistance of mutants was improved by 1-2 levels compared with their parents, and the main agronomic traits such as plant height, spike length and spikelet number also varied. Compared with their parents, the numbers of bands, band intensities and band positions of gliadin and peroxidase isozymes were different among mutants. Activity analysis of superoxide dismutase (SOD) showed that the activity of the mutant was 8,61% ~ 24.14% higher than that of the parent. Mutants have higher superoxide dismutase activity, which is a pathological cause of scab resistance.