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目的探讨胃癌组织中肝素酶和血管内皮生长因子(VEGF)C蛋白表达及其临床意义。方法采用免疫组化SP法检测97例原发性胃癌组织、癌旁组织及20例正常胃黏膜组织中肝素酶和VEGFC蛋白的表达,并分析其与临床病理特征和预后的关系。结果胃癌组织肝素酶和VEGFC蛋白表达阳性率分别为61.9%和66.0%,显著高于癌旁组织的7.2%和5.0%,正常胃组织的8.3%和5.0%(P值均<0.01);肝素酶表达与肿瘤分化程度无关,但与肿瘤直径、淋巴结转移、静脉侵犯、淋巴管侵犯、远处转移、浆膜面受累和TNM分期等密切相关;VEGFC表达与肿瘤直径、分化程度、静脉侵犯及远处转移等无关,但与淋巴结转移、淋巴管侵犯、浆膜面受累和TNM分期等密切相关;肝素酶和VEGFC阳性表达组术后生存率明显低于阴性组;在胃癌中肝素酶和VEGFC阳性表达呈正相关。结论肝素酶及VEGFC蛋白的阳性表达,可作为胃癌预后不良的参考指标。
Objective To investigate the expression of heparinase and vascular endothelial growth factor (VEGF) C protein in gastric carcinoma and its clinical significance. Methods The expression of heparanase and VEGFC protein in 97 cases of primary gastric cancer tissues, adjacent normal tissues and 20 cases of normal gastric mucosa tissues were detected by immunohistochemical SP method. The relationship between them and clinicopathological features and prognosis was analyzed. Results The positive rates of heparanase and VEGFC protein in gastric cancer tissues were 61.9% and 66.0%, respectively, which were significantly higher than those in adjacent tissues (7.2% and 5.0%, 8.3% and 5.0% respectively) (P <0.01). Heparinase expression was not related to the degree of tumor differentiation, but closely related to tumor diameter, lymph node metastasis, venous invasion, lymphatic invasion, distant metastasis, serosal surface involvement and TNM stage. The expression of VEGFC was correlated with tumor diameter, differentiation, Invasion and distant metastasis, but not related to lymph node metastasis, invasion of lymphatic vessels, serosal surface involvement and TNM stage closely related; heparinase and VEGFC positive expression group was significantly lower than the survival rate after surgery; in gastric cancer in the liver The positive expression of VEGFC and VEGFC was positively correlated. Conclusion The positive expression of heparanase and VEGFC protein can be used as a reference index for poor prognosis of gastric cancer.