论文部分内容阅读
目的探讨免疫球蛋白重链(immunoglobulinheavychain,IgH)和T细胞受体(T-cellreceptor,TCR)基因重排在鼻腔非霍奇金淋巴瘤(non-Hodgkinlymphoma,NHL)诊断和分型中的价值。方法采用两对引物进行半巢式聚合酶链反应(polymersechainreaction,PCR)检测11例B细胞淋巴瘤组织DNA的单克隆性IgH基因重排;采用两对引物进行一步法PCR检测23例NK/T细胞淋巴瘤和20例T细胞淋巴瘤组织DNA的单克隆性TCR基因重排。以10例鼻息肉组织标本作为对照。结果11例B细胞淋巴瘤中IgH基因单克隆性重排10例阳性(90·9%),20例T细胞淋巴瘤中TCR基因单克隆性重排17例阳性(85·0%),23例NK/T细胞淋巴瘤中TCR基因单克隆性重排10例阳性(43·5%)。10例鼻息肉标本采用相应引物扩增结果均为阴性。结论在鼻腔NHL中,基因重排检测是一种有效的辅助诊断和分型方法,采用两对引物进行PCR可提高基因重排检测的阳性率。
Objective To investigate the value of immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) gene rearrangements in the diagnosis and classification of nasal non-Hodgkin lymphoma (NHL). Methods Two pairs of primers were used to detect the monoclonal IgH gene rearrangements in 11 cases of B cell lymphoma by polymerase chain reaction (PCR). Two pairs of primers were used to detect 23 cases of NK / T Cell lymphoma and 20 cases of T-cell lymphoma DNA monoclonal TCR gene rearrangement. 10 cases of nasal polyps tissue samples as a control. Results The positive clonality of IgH gene was found in 10 cases (90.9%) in 11 cases of B - cell lymphoma, and 17 cases (85.0%) were positive in 20 cases of T - cell lymphoma. 10 cases of TCR gene monoclonal rearrangement in NK / T cell lymphoma positive (43.5%). 10 cases of nasal polyps specimens using the corresponding primers were negative. Conclusion In nasal NHL, gene rearrangement detection is an effective diagnostic and typing method. PCR using two pairs of primers can improve the detection rate of gene rearrangement.