昆明小鼠30只分别饲以低硒(14.7ng/g)及补硒(500ng/g)等饲料27天,用 ELISA 检测心、肝、肾、肝细胞线粒及胞质 GSH—px 含量,结果表明肝>肾>心,低硒动物组织及肝亚细胞成分酶含量明显下降,补硒则可维持酶含量和活性,本方法灵敏性高,方法简便。低硒时动物组织内 GSH—px 含量下降幅度不及全血酶活性大,揭示低硒早期,动物组织内可能存在无活性蛋白多肽,作者对其发生机制进行了探讨。 30 Kunming mice were fed with low-selenium (14.7ng / g) and supplemental selenium (500ng / g) for 27 days. The contents of mitochondria and cytoplasmic GSH-px in heart, liver, The results showed that the content of enzyme in the liver> kidney> heart, selenium-deficient animal tissues and hepatocyte sub-cellular components decreased significantly. Selenium supplementation could maintain the enzyme content and activity. The method was sensitive and simple. The content of GSH-px in animal tissue decreased less than that of the whole blood enzyme in low selenium, which revealed that there might be inactive protein in early selenium and animal tissues. The mechanism of GSH-