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This paper studied on the way of Coleus blumei,the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine (6-BA),a-naphthaleneacetie acid (NAA) and indole-3-butyric acid (IBA).The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA (2.0 mg·L-1) and NAA (1.0 mg·L-1). Shoot tips were induced on the medium containing 6-BA (4.0 mg·L-1) and NAA (0.5 mg·L-1).The same media conditions were found suitable for shoot multiplication,we multiplied shoots rooted best on 1/2 MS medium supplemented with IBA (0.1 mg·L-1).