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目的 (1)确定北京地区幽门螺杆菌 (Helicobacterpylori,Hp)菌株对克拉霉素的耐药情况。 (2 )研究幽门螺杆菌对克拉霉素耐药与 2 3SrRNA基因点突变的关系。方法 从北京地区 89例有上胃肠症状的患者取得胃活检组织 ,微需氧培养得到Hp ,E 检验方法测定克拉霉素的最低抑菌浓度 (MIC) ,CTAB/NaCl方法提取敏感菌和耐药菌的DNA ,用限制性片段长度多态性 (PCR RFLP)检测克拉霉素耐药菌株的点突变。结果 (1)北京地区Hp菌株对克拉霉素的耐药率是 13 5 %。 (2 ) 12个克拉霉素耐药的Hp菌株均存在 2 3SrRNA基因的A2 143G点突变 ,进行PCR RFLP的 2 4个敏感菌株均无2 3SrRNA的点突变。结论 北京地区克拉霉素耐药的Hp菌株较为常见 ,Hp对克拉霉素的耐药与2 3SrRNA基因的点突变有关。
Objective (1) To determine the resistance of clarithromycin to Helicobacter pylori (Hp) strains in Beijing. (2) To study the relationship between Helicobacter pylori resistance to clarithromycin and point mutation of 23SrRNA gene. Methods Tissue samples of gastric biopsy from 89 patients with upper gastrointestinal symptoms in Beijing were obtained. Micro-aerobic culture was used to obtain Hp. The minimum inhibitory concentration (MIC) of clarithromycin was determined by E method. The sensitive bacteria and resistant strains were extracted by CTAB / NaCl method Bacterial DNA was tested for point mutations in clarithromycin-resistant strains using restriction fragment length polymorphism (PCR RFLP). Results (1) The rate of resistance to clarithromycin in Hp strains in Beijing was 13.5%. (2) A2 143G point mutation of 23SrRNA genes existed in 12 clarithromycin-resistant Hp strains. No point mutation of 23SrRNA was detected in 24 sensitive strains of PCR RFLP. Conclusions Clarithromycin - resistant Hp strains in Beijing are more common. The resistance of Hp to clarithromycin is related to the point mutation of 23SrRNA gene.