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目的探索简便可行的、可以获得高纯度的雪旺细胞的培养方法。方法选用4～5d的SD大鼠将双侧坐骨神经进行结扎预变性,术后7d采用在解剖镜下剥离除去神经外膜,剪碎后采用双酶消化法和原代培养4d后G-418纯化法进行雪旺细胞培养,并对培养的细胞进行细胞计数、活力测定和免疫组织化学染色法鉴定。结果从新生鼠可获取近3.84x106个雪旺细胞,成活率为95.6%,免疫组织化学染色法鉴定雪旺细胞纯度达94%以上。结论建立的方法可以获得大量纯度高、活性良好的雪旺细胞,值得进一步推广。
Objective To explore a simple and feasible method for culturing Schwann cells with high purity. Methods Sprague-Dawley rats were pretreated with ligated sciatic nerve for 4 ~ 5 days. The epineurium was dissected 7 days after operation. The epineurium was removed by dissection and dissection. After double digestion and 4 days of primary culture, G-418 was purified Method for Schwann cell culture, and cultured cells were counted, viability and immunohistochemical staining. Results Nearly 3.84x106 Schwann cells were obtained from neonatal rats, the survival rate was 95.6%. The purity of Schwann cells was above 94% by immunohistochemical staining. Conclusion The established method can obtain a large number of high purity and good activity of Schwann cells, worth further promotion.