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目的分析实验室真菌气溶胶分布特征,为生物实验室工作人员提供预警资料。方法采用6级An-derson空气采样器,马铃薯葡萄糖琼脂(PDA)培养基分别在真菌实验室,普通实验室进行采样。结果真菌实验室的优势菌株为黑曲霉、岛青霉和黄曲霉,分别高于平均浓度195,60和20 CFU/m3;普通实验室的优势菌株为青霉,含量高达67.4%。真菌实验室、普通实验室气溶胶浓度分别为813和304 CFU/m3,计数中值直径(count median dianeter,CMD)分别为2.76和3.66μm,粒子均可直接进入肺泡,而且能直接进入肺泡的真菌粒子浓度是普通实验室的3.1倍。结论真菌实验室真菌浓度远高于普通实验室,已被真菌污染,而普通实验室真菌浓度较低,CMD值与居室接近,因此,拥有较好的空气质量。
Objective To analyze the distribution characteristics of fungal aerosol in laboratory and provide early warning information to biological laboratory staff. Methods Six An-derson air samplers and potato dextrose agar (PDA) medium were used for sampling in fungi laboratory and general laboratory respectively. Results The dominant strains in fungi laboratory were Aspergillus niger, Penicillium island and Aspergillus flavus, which were higher than the average concentration of 195, 60 and 20 CFU / m3, respectively. The dominant strain in common laboratory was Penicillium, with a content of 67.4%. Fungal laboratories, general laboratory aerosol concentrations were 813 and 304 CFU / m3, count median diameter (CMD) were 2.76 and 3.66μm, the particles can be directly into the alveoli, and can directly enter the alveoli The fungal particle concentration is 3.1 times that of the normal laboratory. Conclusion The concentration of fungi in fungi laboratory is much higher than that in common laboratories. It has been contaminated by fungi. However, the concentration of fungi in common laboratories is relatively low and the CMD value is close to that of houses. Therefore, it has better air quality.