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目的 :观察慢性复合应激对大鼠学习与记忆的影响和海马内NMDA受体亚基NR1表达的变化。方法 :成年雄性Wistar大鼠实验组每天交替暴露于复合应激原环境中达 6w ,然后作Morris水迷宫和Y迷宫作业测试 ,再采用免疫组织化学和图像处理方法分析海马CA1、CA3和齿状回区内NR1的表达变化。结果 :慢性复合应激组大鼠寻找平台的潜伏期较对照组明显缩短 ,学会躲避电击的正确次数较对照组明显增多 ;海马内NMDA受体亚基NR1的表达水平较对照组明显上调。结论 :慢性复合应激可增强学习与记忆能力 ,NMDA受体表达变化可能是影响学习与记忆的机制之一
Objective: To observe the effects of chronic multiple stress on learning and memory and the expression of NMDA receptor subunit NR1 in hippocampus of rats. Methods: The experimental group of adult male Wistar rats were exposed alternately to the stress environment of compound stress for 6 weeks, and then were tested by Morris water maze and Y maze. The immunohistochemistry and image processing were used to analyze the changes of CA1, CA3 and dentate Back to the region of NR1 expression changes. Results: The incubation period of rats in chronic stress group was significantly shorter than that in control group, and the correct number of times to avoid electric shock was significantly higher than that in control group. The expression level of NMDA receptor subunit NR1 in hippocampus was significantly higher than that in control group. Conclusion: Chronic multiple stress can enhance learning and memory ability. The change of NMDA receptor expression may be one of the mechanisms that affect learning and memory