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目的 探讨谷胱甘肽转硫酶 (GST)和细胞色素CYP2E1多态性与肺癌易感性的关系。方法 选取广州市广东籍新发肺癌病人 91例及同期非肺部疾患及相同性别的病人 91例作匹配 ,调查他们的吸烟、饮酒等因素的暴露情况。用聚合酶链式反应 (PCR)和限制性片段长度多态性 (RFLP)技术检测CYP2E1和GST的基因多态性。结果 CYP2E1C1C1基因型与C1C2基因型比较 ,其OR为 1.82 (95 %CI =0 .95～ 3.4 0 ) ,GSTM 1基因缺失型的OR值为 1.2 6 (0 .6 9～ 2 .30 ) ,而两者联合分析时 ,则可增加患肺癌的危险 ,其OR值为 2 .13(0 .82～ 5 .5 6 ) ,但无统计学意义 (P >0 .0 5 )。吸烟与肺癌关系密切 ,其OR值为 2 .82(1.5 6～ 5 .12 ) ,当吸烟与这两种基因型协同作用时 ,可明显提高患肺癌的危险性 ,携带CYP2E1C1C1基因型的吸烟者的OR值为 5 .4 2 (2 .0 5～ 14 .32 ) ,GSTM1基因缺失型的吸烟者的OR值为 4 .38(1.81～ 10 .6 1)。多因素logistic回归分析结果表明 :文化程度 (OR为 0 .6 3,0 .4 5～ 0 .86 )、吸烟量 (OR为 1.5 6 ,1.14～ 2 .14 )、无抽油烟机 (OR为 3.77,1.4 8～ 9.5 6 )、食用动物油(OR为 1.6 7,1.2 5～ 2 .2 4 )、胡萝卜 (OR为 0 .4 7,0 .2 2～ 0 .98)、饮酒 (OR为 6 .5 8,1.5 3～ 2 8.3)、直系亲属肺癌史 (OR为 3.75 ,1
Objective To investigate the relationship between glutathione S-transferase (GST) and cytochrome CYP2E1 polymorphisms and lung cancer susceptibility. Methods Ninety-one new-onset lung cancer patients and 91 non-pulmonary disease and same-sex patients in Guangdong during the same period were selected to investigate their smoking, alcohol consumption and other factors. The genetic polymorphisms of CYP2E1 and GST were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results The OR of CYP2E1C1C1 genotype was 1.82 (95% CI = 0.95 ~ 3.4 0) compared with that of C1C2 genotype. The OR of GSTM1 deletion genotype was 1.2 6 (0.69-2.230) The combined analysis of both could increase the risk of lung cancer, with an OR of 2.13 (0.82-5.56), but not statistically significant (P> 0.05). Smoking and lung cancer are closely related, the OR value of 2.82 (1.56 ~ 5.12), when smoking and synergy of these two genotypes can significantly increase the risk of lung cancer, smokers carrying CYP2E1C1C1 genotype OR of 5.42 (2.5-0.14.32), OR of 4.38 (1.81-10.61) in smokers with GSTM1 null genotype. Multivariate logistic regression analysis showed that the educational level (OR was 0.636, 0.45 ~ 0.86), smoking amount (OR 1.56, 1.14 ~ 2.14), no range hood (OR = 1.6 7,1.2 5 ~ 2 .24), carrot (OR = 0.4 7,0 .22 ~ 0 .98) and alcohol consumption .5 8,1.5 3 ~ 2 8.3), immediate family history of lung cancer (OR 3.75,