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目的了解依那普利拉对烧伤后早期心肌损害的防治效果。方法将60只SD大鼠制成30%TBSAⅢ度烫伤模型,分为单纯烫伤组(30只,伤后常规补液)和依那普利拉组(30只,伤后立即一次性腹腔注射依那普利拉1 mg/kg并行常规补液)。另取6只大鼠不致伤作为正常对照组。检测正常对照组及2组烫伤大鼠伤后1、3、6、12、24 h血清心肌肌钙蛋白I(cTnI)的含量、心肌型肌酸激酶同工酶(CK-MB)的活性,并观察心肌组织病理学变化。结果(1)单纯烫伤组大鼠伤后各时相点cTnI、CK-MB均显著高于正常对照组(P<0.01)。依那普利拉组伤后各时相点cTnI为(1.32±0.12)～(2.47±0.22)μg/L,均显著低于单纯烫伤组[(6.42±0.96)～(15.10±3.69)μg/L,P<0.01];其各时相点CK-MB活性为(438±68)～(5569±322)U/L,亦均低于单纯烫伤组[(2556±74)～(8047±574)U/L,P<0.05或P<0.01]。(2)与正常对照组比较,单纯烫伤组伤后出现心肌细胞浊肿、间质血管扩张充血、炎性细胞浸润等病理改变;依那普利拉组病变程度较之减轻。结论大鼠严重烫伤后早期心肌组织受损明显,依那普利拉能显著减轻这些损害。
Objective To understand the prevention and treatment effect of enalapril on early myocardial damage after burn. Methods Sixty SD rats were made into 30% TBSA Ⅲ degree scald models and divided into three groups: simple scald group (30 rats, conventional fluid rehydration) and enalapril group (30 rats) Pilar 1 mg / kg concurrent conventional rehydration). Another 6 rats were not injured as a normal control group. The levels of serum cardiac troponin I (cTnI), the activity of cardiac muscle creatine kinase (CK-MB) and the activity of CK-MB were detected at 1, 3, 6, 12 and 24 hours after injury in normal control group and 2 groups of scalded rats, The changes of myocardial histopathology were observed. Results (1) The levels of cTnI and CK-MB at each time point in scald injury group were significantly higher than those in normal control group (P <0.01). The cTnI of Enalapril group was significantly lower than that of simple burn group [(6.42 ± 0.96) ~ (15.10 ± 3.69) μg / L, L, P <0.01]. The CK-MB activity at each time point ranged from (438 ± 68) to (5569 ± 322) U / L and also lower than that in the scalded group [(2556 ± 74) ~ (8047 ± 574 ) U / L, P <0.05 or P <0.01]. (2) Compared with the normal control group, pathological changes such as myocardial cell turbidity, interstitial vasodilation and congestion and inflammatory cell infiltration appeared in the scald injury group. The lesion degree in enalapril group was lessened. Conclusion Myocardial tissue damage is obvious after severe scald in rats, and enalapril can significantly reduce these damages.