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作者采用CM-Sephadex-C50,Sephadex-G100和DEAE-Sephadex-A50等柱层析方法,从人类精浆中分离纯化精浆特异性抗原p30,经聚丙烯酰胺凝胶电泳、SDS-凝胶电泳与免疫电泳等鉴定证实。
The author used CM-Sephadex-C50, Sephadex-G100 and DEAE-Sephadex-A50 column chromatography method to separate and purify the seminal plasma specific antigen p30 from human seminal plasma. After polyacrylamide gel electrophoresis and SDS-gel electrophoresis And electrophoresis and other identification confirmed.