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目的 :研究高温状态时下丘脑延迟整流钾通道特性的改变 ,探讨高温条件下出现热损伤的作用机制。方法 :对急性分离的新生SD大鼠下丘脑细胞进行高温处理 ,采用细胞贴附式和内面向外式膜片钳记录方法 ,观察通道活动改变情况。结果 :细胞经 4 3℃处理 4 0min后 ,K+通道活动阳性率下降到 38% (n =16 ) ,而对照组 (37℃ )为 86 % (n =36 ,P <0 .0 5) ;4 3℃高温处理6 0min后 ,细胞K+通道活动阳性率下降到 <10 % (n =35) ,而对照组为 78% (n =18,P <0 .0 5) ,并且通道活动呈现多种形式。结论 :高温可能通过对通道蛋白的破坏作用而改变通道活动特性
Objective: To study the changes of delayed rectifier potassium channel in hypothalamus at high temperature and to explore the mechanism of heat injury under high temperature. Methods: Hypothalamic cells were isolated from acutely isolated SD rats. Cell attachment and inward-outward patch-clamp recording were used to observe the change of channel activity. Results: The positive rate of K + channel activity decreased to 38% (n = 16) after treated with 4 3 ℃ for 4 minutes, compared with 86% (n = 36, P <0. 05) in the control group (37 ℃) The positive rate of K + channel activity decreased to <10% (n = 35) at 60 ℃ for 3 min and 78% (n = 18, P <0.05) in the control group, Kind of form CONCLUSION: High temperature may alter the activity of channels through the disruption of channel proteins