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【目的】研究耐辐射短梗霉MF1黑色素分批发酵动力学。【方法】描述短梗霉分批发酵黑色素过程中细胞生长、产物积累、糖消耗的变化规律。基于Logistic方程和Luedeking-Piret方程分别建立黑色素发酵过程菌体生长、黑色素产生及葡萄糖消耗随时间变化的数学模型,对实验值与模型进行了验证比较。【结果】动力学模型计算值结果与实验值拟合良好,较好地反映了短梗霉黑色素分批发酵过程的动力学特征。计算获得菌体最大黑色素生产能力为1.523×10-4g/g葡萄糖·g干菌体·h。【结论】该动力学模型为天然菌株黑色素的中试放大,大规模生产及应用奠定了基础。
【OBJECTIVE】 To study the kinetics of batch fermentation of A. pleuropneumoniae MF1 melanin. 【Method】 The changes of cell growth, product accumulation and sugar consumption during the fermentation of melatonin were compared. Based on the Logistic equation and the Luedeking-Piret equation, a mathematical model of the growth, the production of melanin and the glucose consumption over time in the process of melanin fermentation was established respectively. The experimental values and the model were compared and verified. 【Result】 The results of the kinetic model fit well with the experimental data, which can well reflect the kinetic characteristics of the fermentation process of pullulan. Calculated to obtain the maximum melanin production capacity of bacteria was 1.523 × 10-4g / g glucose · g dry cell · h. 【Conclusion】 The kinetic model lays a foundation for the pilot scale amplification, mass production and application of natural strain melanin.