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目的:观察通冠胶囊对动脉粥样硬化不同阶段血管内皮功能损害的干预情况。方法:健康雄性新西兰大耳白兔36只,随机分为正常对照组,高脂对照组,阿托伐他汀组,通冠胶囊小、中、大剂量组共6组,每组6只,通过免疫性内皮损伤合并高脂饲料诱导法建立动脉粥样硬化兔实验动物模型,高脂对照组喂高脂饲料,阿托伐他汀组在高脂对照组基础上加喂阿托伐他汀5mg/kg/d,通冠胶囊小、中、大剂量组在高脂对照组基础上每天分别加喂通冠胶囊3mg、9mg及15mg,分别于实验开始2、6周末测定血中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、一氧化氮(NO)、内皮素(ET)及假血友病因子(vWF),实验结束后取髂外动脉行血管内皮病理学检测。结果:6周末通冠胶囊中剂量组TC、小剂量组、高脂对照组TC、LDL-C均较2周末显著升高(P<0.05,P<0.01),通冠胶囊大剂量组TC显著降低,同时伴有HDL-C升高(P<0.01)。6周末通冠胶囊大剂量组HDL-C水平明显高于阿托伐他汀组及高脂对照组(P<0.05)。通冠胶囊小剂量组、中剂量组、高脂对照组组内前后比较,vWF、ET水平显著升高(P<0.05),而NO显著降低(P<0.05);通冠胶囊大剂量组、阿托伐他汀组NO显著升高(P<0.05)。6周末通冠胶囊大剂量组与阿托伐他汀组、高脂对照组比较,NO水平明显升高(P<0.05)。组织病理学结果显示,大剂量通冠胶囊与阿托伐他汀均能有效抑制血管内膜动脉粥样斑块的形成。结论:通冠胶囊能够显著提高动脉粥样硬化兔血管内皮因子NO水平,降低vWF及ET水平,从而改善血管的舒缩、凝血及纤溶功能;并能提高血中HDL-C水平及降低TC水平,因而起到抗动脉粥样硬化的作用。
Objective: To observe the effect of Tongguan capsule on vascular endothelial dysfunction at different stages of atherosclerosis. Methods: Thirty-six healthy male New Zealand white rabbits were randomly divided into normal control group, high-fat control group, atorvastatin group, Tongguan capsule small, medium and large dose groups, 6 in each group, Atherosclerotic rabbits were induced by immune endothelial injury combined with high-fat diet induction. The high-fat control group was fed with high-fat diet. Atorvastatin group was given atorvastatin 5 mg / kg on the basis of high-fat control group / d, Tongguan Capsule Small, medium and large dose groups were fed with Tongguan capsule 3mg, 9mg and 15mg daily on the basis of the high fat control group respectively, and the blood total cholesterol (TC) Triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), nitric oxide (NO), endothelin (ET) and von Willebrand factor (vWF) After the experiment, the external iliac artery was taken for examination of vascular endothelial pathology. Results: TC and LDL-C in low dose group and high fat control group were significantly higher than that in the 2nd week (P <0.05, P <0.01) Decreased, accompanied by elevated HDL-C (P <0.01). The level of HDL-C in high-dose Tongguan Capsule group at 6 weeks was significantly higher than that in atorvastatin group and high-fat control group (P <0.05). Tongguan capsule low-dose group, middle dose group, high-fat control group, vWF, ET levels were significantly increased (P <0.05), while NO was significantly lower NO in atorvastatin group increased significantly (P <0.05). Compared with atorvastatin group and high-fat control group, the NO level in the 6-weekend Tongguan capsule high-dose group was significantly increased (P <0.05). Histopathological results showed that high-dose Tongguan capsule and atorvastatin can effectively inhibit the formation of atherosclerotic plaque in atherosclerotic plaque. CONCLUSION: Tongguan Capsule can significantly increase the level of vascular endothelial factor NO in atherosclerotic rabbits and decrease the levels of vWF and ET, thereby improving the vasomotion, coagulation and fibrinolytic functions of blood vessels and increasing the blood levels of HDL-C and TC Level, which play a role in anti-atherosclerotic.