目的　获取小鼠内皮抑素 (endostatin)基因并进行序列测定 ,为今后研究其机制及应用创造条件 .方法　以小鼠肝脏总 RNA为模板 ,用 RT- PCR法从中扩增 endostatin基因 ,并将所得基因重组入 p UC19载体质粒 ,采用自动测序仪及荧光素标记引物 ,测定目的基因序列 .结果　从小鼠肝组织中成功扩增 endostatin基因 ,并成功克隆入 p U C19载体 .经酶切鉴定正确后 ,命名为 p UC- Endo.经测序证实所获基因序列与已知的 endostatin基因序列一致 .结论　成功构建小鼠 endo-statin基因的重组克隆 p U C- Endo,为今后利用 endostatin进行胶质瘤的抗血管生成治疗研究奠定了实验基础 OBJECTIVE: To obtain endostatin gene of mice and to determine the sequence of the endostatin gene for future research.Methods The endostatin gene was amplified by RT-PCR using the total RNA of mouse liver as a template, Gene was recombined into pUC19 vector plasmid and the sequence of the target gene was determined by automatic sequencer and fluorescein-labeled primer.Results The endostatin gene was successfully amplified from mouse liver tissue and successfully cloned into pUC19 vector.After identification by restriction enzyme digestion, , Named as pUC-Endo.The sequencing of the obtained gene was consistent with that of the known endostatin gene.Conclusion The recombinant clone p U C-Endo of mouse endo-statin gene was successfully constructed and used for glioma The anti-angiogenic therapy research laid the foundation for the experiment