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研究目的探讨某些中枢神经系统疾病血清过氧化脂质(LPO)和谷胱甘肽过氧化物酶(GPX)水平的变化。处理方法 47例脑梗塞患者和14例急性一氧化碳中毒后迟发性脑病(DEACMP)患者均在发病一周内抽取空腹静脉血,迅速分离血清,置-20℃冰箱保存,3天内测定。对照组为42例健康人。LPO和GPX测定均采用荧光分光光度法。统计学处理采用t检验。结果脑梗塞患者和DEACMP患者血清LPO明显高于对照组(P<0.01),GPX明显低于对照组(P<0.01)。两组患者LPO/GPX的比值明显高于对照组(P<0.01),且较LPO增高更为明显。结论脑梗塞患者和DEACMP患者血青LPO增高,GPX降低。表明脑缺血或缺氧后脑组织的损伤与自由基引发的脂质过氧化作用增强有关。LPO/GPX的比值作为过氧化损害的检测指标较LPO更为敏感。
Objective To investigate the changes of serum lipid peroxides (LPO) and glutathione peroxidase (GPX) in some central nervous system diseases. Methods 47 patients with cerebral infarction and 14 patients with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) patients were taken fasting venous blood within a week of onset, rapid separation of serum, stored at -20 ℃ refrigerator, measured within 3 days. The control group was 42 healthy people. LPO and GPX determination using fluorescence spectrophotometry. Statistical analysis using t test. Results Serum LPO of patients with cerebral infarction and DEACMP was significantly higher than that of the control group (P <0.01), and GPX was significantly lower than that of the control group (P <0.01). LPO / GPX ratio was significantly higher in both groups (P <0.01), and more obvious than LPO. Conclusion Serum LPO increased and GPX decreased in patients with cerebral infarction and DEACMP. This suggests that the damage of brain tissue after cerebral ischemia or hypoxia is related to the increase of free radical-induced lipid peroxidation. The LPO / GPX ratio is more sensitive than LPO as a measure of peroxidative damage.