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目的:检测RECK基因及MMP-2、MMP-9在不同前列腺细胞株BPH-1、DU-145、LNCap以及PC-3中的表达,探讨其表达差异及其意义。方法:应用RT-PCR检测4种不同前列腺细胞株RECK及MMP-2、MMP-9 mRNA的表达;应用Western印迹法检测不同前列腺细胞株中RECK蛋白的表达。结果:前列腺癌细胞株DU-145、LNCap以及PC-3中RECKmRNA的表达较良性前列腺增生细胞株BPH-1中明显降低(P<0.01),MMP-2、MMP-9 mRNA表达则明显升高(P<0.01)。另外,DU-145、LNCap以及PC-3细胞株中RECK蛋白的表达较正常组织明显降低(P<0.01)。结论:RECK基因在前列腺癌中的表达量明显下降,而MMP-2、MMP-9的表达量明显升高,RECK基因可能通过抑制MMP-2、MMP-9的表达起到抑癌基因的作用。
OBJECTIVE: To detect the expression of RECK gene and MMP-2, MMP-9 in different prostate cell lines BPH-1, DU-145, LNCap and PC-3, and to explore the differences and their significance. Methods: The expression of RECK, MMP-2 and MMP-9 mRNA in 4 different prostate cell lines was detected by RT-PCR. The expression of RECK protein in different prostate cell lines was detected by Western blotting. Results: The expression of RECK mRNA in prostate cancer cell lines DU-145, LNCap and PC-3 was significantly lower than that in benign prostatic hyperplasia cell line BPH-1 (P <0.01), while the expression of MMP-2 and MMP-9 mRNA was significantly increased (P <0.01). In addition, the expression of RECK protein in DU-145, LNCap and PC-3 cell lines was significantly lower than that in normal tissues (P <0.01). Conclusion: The expression of RECK gene in prostate cancer significantly decreased, while the expression of MMP-2 and MMP-9 was significantly increased. RECK gene may play a role in tumor suppressor gene by inhibiting the expression of MMP-2 and MMP-9 .