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目的 探讨血管紧张素转换酶 (ACE)基因I/D多态性与原发性高血压 (EH)及高血压血栓前状态 (PTS)的关系。方法 PCR检测 6 1例原发性高血压病人和正常对照组 2 8例的ACE基因I/D多态性 ;发色底物法测t PA、PAI 1活性 ,酶联免疫吸附双抗夹心法 (ELISA)测vWF含量。结果 高血压组DD基因型频率显著高于对照组 (P <0 0 5 ) ,但D等位基因频率分布在高血压组和正常组之间差异无显著意义 (P >0 0 5 )。高血压组t PA活性降低 ,PAI 1活性、vWF含量升高 (P均 <0 0 0 1)。高血压组DD型t P活性明显低于ID、II型 (P <0 0 0 1) ,而ID、II型之间差异无显著意义 (P >0 0 5 ) ,DD型PAI 1活性明显高于ID、II型(P <0 0 0 1) ,而ID、II型之间差异无显著意义 (P >0 0 5 ) ,vWF在DD、ID、II型三者之间差异无显著意义 (P>0 0 5 )。结论 DD型是原发性高血压发病的危险因素。原发性高血压存在血栓前状态。t PA、PAI 1的变化与血管紧张素转换酶基因I/D多态性有关 ,DD基因型可引起血栓前状态。
Objective To investigate the relationship between angiotensin converting enzyme (ACE) gene I / D polymorphism and essential hypertension (EH) and prethrombotic state of hypertension (PTS). Methods The ACE gene I / D polymorphism was detected by PCR in 28 cases of patients with essential hypertension and 28 cases of normal control. The t PA and PAI 1 activities were assayed by chromogenic substrate assay. The enzyme linked immunosorbent assay (ELISA) to measure the content of vWF. Results The frequency of DD genotype in hypertension group was significantly higher than that in control group (P <0.05). However, there was no significant difference in the frequency of D allele between hypertensive group and normal group (P> 0.05). In the hypertensive group, t PA activity decreased, PAI 1 activity and vWF content increased (all P <0 0 01). The t-P activity of DD type in hypertensive group was significantly lower than that of ID and type II (P <0.01), while no significant difference was found between type ID and type II (P> 0.05), and the activity of DD type PAI1 was significantly higher There was no significant difference between ID and type II (P <0.01), but there was no significant difference between type ID and type II (P> 0.05) P> 0 0 5). Conclusion DD type is the risk factor of essential hypertension. Pre-thrombotic conditions exist in essential hypertension. t PA, PAI 1 changes and angiotensin-converting enzyme gene I / D polymorphism, DD genotype can cause prethrombotic state.