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目的:探讨咖啡酸苯乙酯(caffeic acid phenethyl ester, CAPE)对体外培养的大肠癌细胞HCT116细胞增生、细胞周期和凋亡的影响. 方法:不同浓度CAPE处理体外培养的HCT116细胞后,采用MTT法检测处理后24、48、72、96 h HCT116细胞的增生活性;PI染色、流式细胞仪检测处理后24 h细胞周期分布;Annexin V-FITC/PI双染色、流式细胞仪检测处理后24 h细胞凋亡率. 结果:80,40,20,10,5.0,2.5 mg/L CAPE处理HCT116细胞24,48,72,96 h后,细胞增生明显受到抑制,呈时间和剂量依赖性特点.流式细胞仪细胞周期分析表明,10,5.0,2.5 mg/L CAPE处理HCT116细胞24 h后,G0/G1期细胞百分率上升,S期细胞百分率下降,呈剂量依赖性.流式细胞仪细胞凋亡率分析表明: 10,5.0,2.5 mg/L CAPE处理HCT116细胞24 h后, 细胞凋亡率上升,呈剂量依赖性. 结论:CAPE对HCT116细胞具有明显的增生抑制作用,其机制与其阻滞细胞周期和诱导凋亡有关.
Objective: To investigate the effect of caffeic acid phenethyl ester (CAPE) on proliferation, cell cycle and apoptosis of human colorectal cancer cell line HCT116 in vitro.METHODS: HCT116 cells cultured in vitro with different concentrations of CAPE were treated with MTT Proliferation activity of HCT116 cells was detected at 24, 48, 72, 96 h after treatment. Cell cycle distribution was detected by PI staining and flow cytometry at 24 h. Annexin V-FITC / PI double staining and flow cytometry 24 h apoptosis rate.Results: After treated with 80, 40, 20, 10, 5.0 and 2.5 mg / L CAPE for 24,48,72,96 h, the proliferation of HCT116 cells was obviously inhibited in a time-and dose-dependent manner Flow Cytometry Cell cycle analysis showed that the percentage of cells in G0 / G1 phase increased and the percentage of S phase cells decreased in a dose-dependent manner after HCT116 cells were treated with 10,5.0,2.5 mg / L CAPE for 24 h. Flow cytometry The results of apoptosis analysis showed that the apoptosis rate of HCT116 cells treated with 10,5.0,2.5 mg / L CAPE for 24 h increased in a dose-dependent manner.CONCLUSION: CAPE can significantly inhibit the proliferation of HCT116 cells in a dose-dependent manner Retarded cell cycle and induction of apoptosis.