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目的:观察中药益肾调肝汤Ⅱ号方对PCOS模型大鼠卵巢的影响,并试图探求治疗机理。方法:24日龄Wistar大鼠进行皮下埋植左炔诺孕酮(左旋18-甲基炔诺酮硅胶棒)结合皮下注射hCG造模,分组灌服生理盐水、二甲双胍、中药后测定各组大鼠血清雄激素等性激素水平,并观察卵巢病理形态组织学变化。结果:运用中药益肾调肝汤Ⅱ号方中高剂量后,血清FSH、E2升高而LH、T明显下降,缩小或减少卵巢扩张囊泡,使卵泡结构转清晰,增加发育卵泡数,减少不成熟的早期发育卵泡,增加黄体数量。结论:益肾调肝汤Ⅱ号方可调节下丘脑-垂体-卵巢轴,纠正卵巢的病理改变,促进排卵,恢复卵巢功能。
Objective: To observe the effect of Yishen Tiaogan Decoction Ⅱ on the ovary in PCOS rat model, and to explore the therapeutic mechanism. Methods: 24-day-old Wistar rats were implanted subcutaneously with levonorgestrel (levonorgestrel-18) and subcutaneously injected with hCG. The rats were divided into three groups: normal saline, metformin, Serum androgen and other sex hormones, and observe the ovarian histopathological changes. Results: After using middle and high dose of Yishen Tiaogan Decoction Ⅱ, serum FSH and E2 increased, LH and T decreased obviously, reduced or reduced ovarian dilatation vesicles, cleared the follicular structure, increased the number of developing follicles and decreased Mature early follicle development, increase the number of corpus luteum. Conclusion: Yishen Tiaogan Decoction Ⅱ can regulate the hypothalamus - pituitary - ovarian axis, correct the pathological changes of ovary, promote ovulation and restore ovarian function.