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为明确抗性生物型荠菜对苯磺隆的抗性机制,分别测定了苯磺隆对抗性和敏感生物型荠菜体内乙酰乳酸合成酶(ALS)、谷胱甘肽-S-转移酶(GSTs)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的影响。结果表明:离体条件下,抗性生物型荠菜体内ALS对苯磺隆的敏感性明显降低,苯磺隆对荠菜抗性和敏感生物型ALS的抑制中浓度(I50)分别为0.722 8和0.052 1μmol/L,抗性与敏感生物型I50的比值为13.87;活体条件下,施用苯磺隆后,抗性和敏感生物型荠菜ALS活性均受到一定程度的抑制,但抗性生物型ALS活性受到抑制后能逐渐恢复,而敏感生物型则不能恢复;经苯磺隆处理后,抗性生物型GSTs相对活力明显高于敏感生物型,而抗性和敏感生物型体内POD、SOD和CAT相对活力无明显差异。研究表明,抗性生物型荠菜体内ALS对苯磺隆敏感性降低是其抗药性产生的原因之一,而GSTs对苯磺隆代谢能力的差异也可能与荠菜对苯磺隆的抗性有关。
In order to clarify the mechanism of resistance to tribenuron-methyl in resistant biotype Shepherd’s pursee, the contents of acetolactate synthase (ALS), glutathione-S-transferase (GSTs) , Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT). The results showed that the sensitivity of trifoliate-resistant ALS to tribenuron-methyl was significantly reduced in vitro and the I50 concentrations of tribenuron-methyl-resistant and susceptible ALS were 0.722 8 and 0.052 1μmol / L, and the ratio of resistance to sensitive biological type I50 was 13.87. Under the living conditions, the activity of ALS of resistant and sensitive biotype Shepherd’s’s Pursee was inhibited to a certain degree, but the activity of resistant biological ALS was affected After the inhibition, it gradually recovered, while the sensitive biotypes could not recover. After treated with tribenuron-methyl, the relative bioactivities of resistant biotype GSTs were significantly higher than those of sensitive biotypes, while the relative bioactivities of POD, SOD and CAT in resistant and sensitive biotypes were relatively high No significant difference. Studies have shown that the decrease of sensitivity to tribenuron-methyl in the resistant biological herd of Sheep’s pursee is one of the causes of its resistance. However, the difference in metabolism of triptolide by GSTs may also be related to the resistance of shepherd’s purse to tribenuron-methyl.