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目的探讨ELISA和免疫印迹(IBT)法检测GADAb的差异。方法分别采用糖尿病自身抗体免疫印迹试剂盒、Euroimmune ELISA试剂盒和放射配体结合(RBA)法检测118例新诊断糖尿病患者血清中的GADAb,比较3种方法的检测结果,以及IBT和Euroimmune ELISA试剂盒与RBA的一致性。结果 GADAb的阳性检出率:RBA(13.6%)=ELISA(13.6%)>IBT(5.1%)。与RBA法的一致性:ELISA(98.3%.Kappa值为0.928)>IBT(91.5%,Kappa值为0.509)。以RBA作为诊断标准,ELISA的曲线下面积(AUC)为0.968。Euroimmun ELISA试剂盒检测GADAb的浓度与RBA的GADAb-Index呈正相关(r=0.985)。结论 Euroimmune ELISA试剂盒检测GADAb的效果优于IBT,与RBA相当。
Objective To investigate the differences of GADAb between ELISA and Western blot (IBT). Methods Serum GADAb was detected in 118 newly diagnosed diabetic patients by using the autoantibodies against diabetes mellitus (BLAST), Euroimmune ELISA kit and radioligand binding assay (RBA). The results of three methods were compared with those of IBT and Euroimmune ELISA Consistency of box and RBA. Results The positive detection rate of GADAb: RBA (13.6%) = ELISA (13.6%)> IBT (5.1%). Consistency with RBA: ELISA (98.3% .Kappa value of 0.928)> IBT (91.5%, Kappa value of 0.509). Using RBA as a diagnostic standard, the area under the curve (AUC) for ELISA was 0.968. The concentration of GADAb detected by Euroimmun ELISA kit was positively correlated with GADAb-Index of RBA (r = 0.985). Conclusion The Euroimmune ELISA kit is superior to IBT in detecting GADAb, which is equivalent to RBA.