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应用LSAB免疫组织化学方法检测74例大肠癌中nm23－H1、p53、PCNA以及Ⅳ型胶原的表达情况，并探讨它们的表达与肿瘤浸润转移的关系。结果显示大肠癌中nm23－H1、p53、PCNA的阳性率分别为71．6％、52．7％、81．1％。nm23－H1低表达与淋巴结转移有关（P＜0．025），在Ⅳ型胶原表达不同的肠癌中nm23－H1的表达无明显差异；p53、PCNA过表达与浸润程度以及淋巴结转移有关（P＜0．05）。提示nm23－H1低表达仅作用于大肠癌的转移过程，可作为预测大肠癌转移的较好指标。p53过表达在浸润转移过程及细胞增殖中均起重要作用。
LSAB immunohistochemistry was used to detect the expression of nm23-H1, p53, PCNA, and type IV collagen in 74 cases of colorectal cancer, and to explore the relationship between their expression and tumor invasion and metastasis. The results showed that the positive rates of nm23-H1, p53 and PCNA in colorectal cancer were 71.6%, 52.7% and 81.1%, respectively. The low expression of nm23-H1 was associated with lymph node metastasis (P<0.025). There was no significant difference in the expression of nm23-H1 between different types of colorectal cancers. The overexpression of p53 and PCNA was related to the degree of invasion and lymph node metastasis (P <0.05). This suggests that the low expression of nm23-H1 only affects the metastasis of colorectal cancer and can be used as a better predictor of metastasis of colorectal cancer. Overexpression of p53 plays an important role in invasive metastasis and cell proliferation.