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目的探讨乳腺癌中胰岛素样生长因子-2(IGF-2)的印迹状态与乳腺癌发生发展的关系。方法采用聚合酶链反应(PCR)和限制性片断长度多态性(RFLP)的方法,在47例乳腺癌组织中筛选IGF-2杂合标本,并用逆转录PCR-RFLP检测杂合标本IGF-2的印迹状态。结果在癌组织和癌旁组织中都发现了IGF2的印迹丢失,癌旁组织中IGF2的印迹丢失率(79.2%)比癌组织中的发生率(50.0%)高,两组的差异有统计学意义(P=0.035)。如果乳腺癌组织中发现有IGF-2印迹丢失,其对应的癌旁组织也发现了IGF-2的印迹丢失,但如果乳腺癌组织中为IGF-2印迹保持,则其对应的癌旁组织有的为IGF-2印迹保持,有的为IGF-2印迹丢失。结论IGF2的印迹丢失可能参与了乳腺癌的发展过程。
Objective To investigate the relationship between the imprinting status of insulin-like growth factor-2 (IGF-2) and the development of breast cancer in breast cancer. Methods 47 cases of breast cancer tissues were screened by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). IGF-2 heterozygous specimens were screened by reverse transcription PCR-RFLP. 2 imprinted state. Results Loss of imprinting of IGF2 was found in both cancerous and paracancerous tissues. The loss of imprinting of IGF2 in paracancerous tissues (79.2%) was higher than that in cancerous tissues (50.0%). The difference between the two groups was statistically significant Meaning (P = 0.035). If IGF-2 imprinting is found to be missing in breast cancer tissue, imprinting loss of IGF-2 is also detected in its corresponding paracancerous tissue, but if IGF-2 imprinting is maintained in breast cancer tissue, its corresponding paracancerous tissue is For IGF-2 blotting, and some for IGF-2 blot loss. Conclusion The loss of IGF2 imprinted may be involved in the development of breast cancer.