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目的:利用RAPD和ISSR分子标记技术对来自23个主要分布区的冬凌草进行遗传多样性和亲缘关系分析。方法:从48条RAPD引物中筛选出10条多态性引物,对供试材料进行PCR扩增后共获得84条条带,其中多态性条带77条(PPB=91.67%),23份材料间的遗传相似系数(GS)为0.71~1.00;从70条ISSR引物中筛选出5条多态性引物,对供试材料进行PCR扩增后共获得21条条带,其中多态性条带16条(PPB=76.19%),23份材料间的遗传相似系数(GS)为0.55~1.00。结果:聚类分析显示,不同地区冬凌草基因水平的分类具有较强的地域性。分布于伏牛山的冬凌草明显聚为一类,分布于太行山的冬凌草则聚为另一类。两种标记结果呈显著相关性,相关系数为0.636 5。结论:我国冬凌草植物的遗传多样性十分丰富,该研究为筛选优质种质资源提供了丰富的遗传基础。
OBJECTIVE: To analyze the genetic diversity and genetic relationship of Rubescens from 23 major distribution areas using RAPD and ISSR molecular markers. Methods: Ten polymorphic primers were screened from 48 RAPD primers. A total of 84 bands were obtained after PCR amplification, of which 77 were polymorphic bands (PPB = 91.67%), 23 The genetic similarity coefficient (GS) between the two materials was 0.71-1.00. Five polymorphic primers were screened from 70 ISSR primers, and 21 bands were obtained after PCR amplification of the tested materials. Among them, the polymorphic bands With 16 bands (PPB = 76.19%). The genetic similarity coefficient (GS) of 23 materials was 0.55-1.00. Results: Cluster analysis showed that the classification of Rubescens indica gene in different regions had strong regional characteristics. Rubescens distributed in the Funiu Mountains clearly clustered into one group, distributed in the Taihang Mountains Rubescens gathered for another class. There was a significant correlation between the two markers, with a correlation coefficient of 0.636 5. Conclusion: The genetic diversity of Rubescens plant in China is very rich. This study provides a rich genetic basis for screening high quality germplasm resources.