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目的:观察骨髓间充质干细胞(BMSC)移植对脑梗死大鼠神经功能恢复的影响,并对其相关机制进行探讨。方法:90只大鼠随机分为3组:假手术组、对照组、BMSC移植组,每组30只。对照组和BMSC移植组建立大鼠大脑中动脉阻塞(MCAO)模型,假手术组只需要分离大鼠颈部组织,而不造MCAO模型。BMSC移植组在MCAO模型术后1天经尾静脉注射1 mL/3×10~6 BMSC,对照组注射同剂量的生理盐水,于MCAO术后1 d、3 d、7 d、14 d、21 d、28 d、35 d、42 d、49 d分别对各组大鼠进行神经功能评分(mNSS),术后2个月对BMSC移植组及对照组大鼠脑组织进行免疫组化染色,检测MAP2、TUJ1、Ⅷ因子、GFAP的表达情况。结果:在治疗后的第7天至第35天,BMSC移植组mNSS均显著低于对照组(P<0.05)。术后2个月,BMSC移植组MAP2、TUJ1、Ⅷ因子表达量显著高于对照组,而GFAP表达量显著低于于BMSC对照组(P<0.01)。结论:BMSC移植可以促进脑梗死神经功能的恢复。
Objective: To observe the effect of bone marrow mesenchymal stem cell (BMSC) transplantation on the recovery of neural function after cerebral infarction in rats, and to explore its mechanism. Methods: Ninety rats were randomly divided into three groups: sham operation group, control group and BMSC transplantation group, 30 rats in each group. The control group and BMSC transplantation group established middle cerebral artery occlusion (MCAO) model in rats, sham operation group only need to isolate rat neck tissue, but not make MCAO model. The BMSC transplantation group was injected with 1 mL / 3 × 10 ~ 6 BMSC via the caudal vein 1 day after MCAO and the control group with the same dose of saline. The rats in the control group were injected with 1, 3, 7, 14, 21 Neurological function scores (mNSS) were performed on the rats in each group on day 28, day 35, day 42 and day 49 respectively. The brain tissues of the rats in the BMSC transplantation group and the control group were examined by immunohistochemistry at 2 months after operation. MAP2, TUJ1, Ⅷ factor, GFAP expression. Results: From the 7th day to the 35th day after treatment, the mNSS in the BMSC transplantation group was significantly lower than that in the control group (P <0.05). At 2 months after operation, the expression of MAP2, TUJ1 and factor Ⅷ in BMSC transplantation group was significantly higher than that in control group, while the GFAP expression level was significantly lower than that in BMSC control group (P <0.01). Conclusion: BMSC transplantation can promote the recovery of neurological function after cerebral infarction.