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目的 探讨外源性碱性成纤维生长因子 (basicfibroblastgrowthfactor ,bFGF)对心肌梗塞血管生成的作用。方法 2 4只犬随机分成对照组 (心梗区注射生理盐水 )和实验组 (心梗区注射bFGF)。每组观察 4个不同的时间点 (1d、3d、10d、17d)。各组分别在术后 3h内及处死前行MR心肌灌注成像。免疫组织化学方法检测各组心肌细胞中血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF)的表达及微血管数量。结果 微血管的数量除第 1天外各个时间点实验组比对照组明显增多 ;VEGF的表达除第 17天外各时间点实验组比对照组明显增强 ;TTC染色和MR心肌灌注成像 2种方法显示心梗范围对照组和实验组各个时间点之间无显著性差异。结论 局部心肌内注射bFGF有促进心梗区域新生血管形成、减小心梗范围的作用 ;VEGF的表达与组织中微血管的密度密切相关 ;MR心肌灌注成像可有效评价心肌血流灌注和定量评价心肌梗死的范围。
Objective To investigate the effect of basic fibroblast growth factor (bFGF) on angiogenesis in myocardial infarction. Methods 24 dogs were randomly divided into control group (injection of normal saline in myocardial infarction area) and experimental group (injection of bFGF in myocardial infarction area). Each group observed 4 different time points (1d, 3d, 10d, 17d). All groups were performed MR myocardial perfusion imaging within 3h after operation and before death. Immunohistochemistry was used to detect the expression of vascular endothelial growth factor (VEGF) and the number of microvessels in each group of cardiomyocytes. Results The numbers of microvessels in experimental group were significantly higher than those in control group except for the first day. The expression of VEGF in experimental group was significantly higher than that in control group except for the 17th day. TTC staining and MR perfusion imaging showed that myocardial infarction There was no significant difference between the control group and the experimental group at each time point. Conclusions Local intramyocardial injection of bFGF can promote the formation of angiogenesis in myocardial infarction area and reduce the infarct size. The expression of VEGF is closely related to the density of microvessels in the tissue. MR perfusion imaging can effectively evaluate the myocardial perfusion and quantitatively evaluate myocardial The area of infarction.