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为了研究白血病患者体内是否有NUP98 HOXA、NUP98 HOXB、NUP98 HOXC、NUP98 HOXD融合基因 ,提取了骨髓中单个核细胞总RNA ,用甲醛变性电泳检测其完整性 ;反转录合成cDNA第一链并设计了 17对引物 ,以巢式PCR法 (nested PCR )扩增NUP98 HOXA融合基因 ;一步法扩增NUP98 HOXB、NUP98 HOXC和NUP98 HOXD融合基因 ,4 12bpGAPDH作为内参照引物 ,2 %琼脂糖凝胶电泳判断PCR结果。结果表明 :所提RNA完整 ,反转录后PCR扩增每个样本中均有内参照GAPDH的表达 ,但是未发现有NUP98 HOXA、NUP98 HOXB、NUP98 HOXC、NUP98 HOXD融合基因。结论 :在本研究所取新疆白血病人群中没有检测到NUP98 HOXA、NUP98 HOXB、NUP98 HOXC和NUP98 HOXD融合基因。
In order to study whether there are NUP98 HOXA, NUP98 HOXB, NUP98 HOXC and NUP98 HOXD fusion genes in leukemia patients, the total RNA of bone marrow mononuclear cells was extracted and the integrity of mononuclear cells was detected by formaldehyde denaturing electrophoresis. The first strand of cDNA was reverse transcribed and designed 17 pairs of primers were used to amplify the NUP98 HOXA fusion gene by nested PCR. NUP98 HOXB, NUP98 HOXC and NUP98 HOXD fusion genes were amplified in one step, 4 12bpGAPDH was used as internal reference primer, and 2% agarose gel electrophoresis Judge PCR results. The results showed that the internal reference GAPDH was expressed in each sample by PCR amplification, but NUP98 HOXA, NUP98 HOXB, NUP98 HOXC and NUP98 HOXD fusion genes were not found. Conclusion: No NUP98 HOXA, NUP98 HOXB, NUP98 HOXC and NUP98 HOXD fusion genes were detected in the population of Xinjiang leukemia.