目的 :检测手术中取出的增生性玻璃体视网膜 (PVR )病变患者的视网膜前膜 (ERM )组织及培养的人视网膜色素上皮细胞 (RPE )中的C5a受体 (C5aR)的表达及分布 ,阐明C5a受体在发生PVR的病理过程中可能的作用。方法 :手术取出ERM组织 ,常规制备石腊切片。另取新鲜人眼球 ,常规分离、培养人RPE细胞。以小鼠抗人C5aR单克隆抗体作为检测抗体 ,对PVR患者的ERM组织及培养的人RPE细胞做细胞免疫化学ABC染色 ,检测C5aR的表达。结果 :PVR患者的ERM组织及培养的人RPE细胞上均有C5aR的表达。结论 :C5a作为一种前炎性因子 ,与RPE细胞上的C5aR结合后 ,可能介导PVR的发生 ,即PVR的发生可能与C5a有关。 OBJECTIVE: To detect the expression and distribution of C5a receptor (C5aR) in the preoptic membrane (ERM) tissue and cultured human retinal pigment epithelium (RPE) in patients with proliferative vitreoretinopathy (PVR) Receptors may play a role in the pathogenesis of PVR. Methods: The ERM tissues were removed surgically and paraffin sections were prepared routinely. Another fresh eye, routine separation, culture of human RPE cells. Mouse anti-human C5aR monoclonal antibody was used as the detection antibody to perform immunohistochemical ABC staining on ERM tissues and cultured human RPE cells in PVR patients to detect the expression of C5aR. Results: The expression of C5aR was found in both ERM tissues and cultured human RPE cells in patients with PVR. Conclusion: C5a as a proinflammatory cytokine may bind with C5aR on RPE cells and may mediate the occurrence of PVR. That is, the occurrence of PVR may be related to C5a.