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目的研究磁力介导的骨髓间充质干细胞(BMSCs)对糖尿病大鼠创面的靶向修复作用。方法采用超顺磁性氧化铁纳米颗粒(SPIONs)标记原代培养的BMSCs,普鲁士蓝染色评价标记效果,MTT法绘制细胞生长曲线,以活细胞染色剂CM-Dil对干细胞进行双标记。制作糖尿病大鼠创面模型,随机分为实验组及对照组,每组10只,两组均采用SPIONs标记的BMSCs治疗,实验组创面局部施加磁场,对照组无磁场作用。评价创面组织BMSCs聚集效果,HE染色观察创面愈合情况。结果普鲁士蓝染色结果显示,SPIONs标记率>95%;细胞生长曲线表明,标记后细胞与未标记细胞生长曲线无统计学差异(P>0.05)。CM-Dil标记后显示实验组创面组织BMSCs聚集多于对照组;HE染色显示实验组创面较对照组创面组织结构更清晰、上皮化程度更高。结论 SPIONs标记后BMSCs在磁场作用下能向糖尿病大鼠创面聚集并促进创面愈合。
Objective To investigate the effect of magnetic-mediated bone marrow mesenchymal stem cells (BMSCs) on wound healing in diabetic rats. Methods Primary cultured BMSCs were labeled with SPIONs. Prussian blue staining was used to evaluate the labeling effect. Cell growth curve was drawn by MTT method and double labeled with live cell stain CM-Dil. The wounds of diabetic rats were made and randomly divided into experimental group and control group, with 10 rats in each group. Both groups were treated with SPIONs-labeled BMSCs. Magnetic field was applied to the wounds in the experimental group and no magnetic field in the control group. Evaluation of wound tissue BMSCs aggregation effect, HE staining observed wound healing. Results The results of Prussian blue staining showed that the SPIONs labeling rate was> 95%. The cell growth curve showed that there was no significant difference between the labeled and unlabeled cells (P> 0.05). After CM-Dil labeling, there were more BMSCs in the experimental group than those in the control group. The HE staining showed that the wound tissue in the experimental group was clearer and the degree of epithelization was higher than that in the control group. Conclusion After BMSCs were labeled with SPIONs, BMSCs can accumulate in wounds of diabetic rats and promote wound healing under the action of magnetic field.