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利用PCR技术从大豆品种东农42基因组中分离得到了GmGAMYB1基因启动子片段pGmGAMYB1,定向替换载体pBI121的CAMV35S组成型启动子,构建表达载体pBI121-pGmGAMYB1,驱动下游报告基因GUS基因表达,并利用PLACE和PlantCARE在线启动子预测工具进行分析。结果表明:序列中含有多种典型的光及各种激素和胁迫诱导特异性表达元件,如光应答元件如3-AF1 binding site、BOX-4、T-Box和TCT-motif;赤霉素应答元件GARE;脱落酸应答元件ABRE;热激元件CCAATBox;节律钟Ciradian等。
The GmGAMYB1 gene promoter fragment pGmGAMYB1 was isolated from the soybean cultivar Dongnong42 by PCR and the CAMV35S constitutive promoter of the vector pBI121 was replaced by the vector to construct the expression vector pBI121-pGmGAMYB1 to drive the downstream gene GUS gene expression. PLUS And PlantCARE online promoter prediction tools for analysis. The results showed that the sequences contained a variety of typical light and various hormones and stress-induced specific expression elements such as light-responsive elements such as 3-AF1 binding site, BOX-4, T-Box and TCT- Element GARE; abscisic acid response element ABRE; heat shock element CCAATBox; rhythm clock Ciradian et al.