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目的为了解吉林省2013年肠道病毒71型(EV71)流行株基因学特征。方法对吉林省2013年手足口病来源的EV71阳性分离物进行VP1编码区逆转录-聚合酶链反应(RT-PCR)扩增,并对扩增产物进行核苷酸序列测定,使用Mega4和Bioedit软件对扩增序列进行基因亲缘关系和氨基酸位点变异分析。结果吉林省2013年分离到的7株EV71同属于C4a基因亚型,并且在VP1编码区第22位氨基酸位点均由谷氨酰胺(Q)突变为组氨酸(H),这一结果与2008年安徽阜阳株突变一致;有2株在VP1编码区第289位点由丙氨酸(A)突变为缬氨酸(V)。结论吉林省2013年的7株EV71流行株与C4a代表株位于同一分支,属于C4a基因亚型,且与2008年阜阳株(EU703813-Fuyang-08/2-C4a)亲缘关系最近,而且发生了氨基酸位点变异。
Objective To understand the genetic characteristics of enterovirus 71 (EV71) epidemic strains in Jilin Province in 2013. Methods EV71 positive isolates from hand, foot and mouth disease in Jilin Province were amplified by reverse transcription-polymerase chain reaction (RT-PCR) in VP1 coding region and the nucleotide sequences were determined. Mega4 and Bioedit The software analyzes the genetic relationship and amino acid site variation of amplified sequences. Results The seven EV71 isolates from Jilin Province in 2013 belonged to C4a subtype and mutated from glutamine (Q) to histidine (H) at amino acid position 22 of VP1 coding region, In 2008, Fuyang strains in Anhui Province were identical to each other. Two of them mutated from alanine (A) to valine (V) at position 289 of VP1 coding region. Conclusions Seven strains of EV71 in Jilin Province were located on the same branch with the C4a strain in 2013 and belonged to the C4a genotype, and were most closely related to the 2008 Fuyang strain (EU703813-Fuyang-08/2-C4a). Amino acids Site variation.