论文部分内容阅读
目的:研究巴氯芬及β-环糊精的包结作用和巴氯芬及β-环糊精包结物与牛血清白蛋白的相互作用。方法:用紫外可见分光光度法与核磁共振法研究了β-环糊精对巴氯芬的包结行为;用摩尔比法确定了包结物的化学计量比;用公式计算了不同温度下巴氯芬与β-环糊精的包结常数;用荧光光谱法研究了巴氯芬与包结物对牛血清白蛋白牛血清白蛋白的相互作用。结果:巴氯芬与β-环糊精的包结过程是自发的,主要驱动力为疏水作用力;巴氯芬及包结物都会对牛血清白蛋白产生静态猝灭。结论:巴氯芬与β-环糊精的包结比为1∶1;巴氯芬与牛血清白蛋白的主要作用为疏水作用,而包结物与牛血清白蛋白主要为静电作用,这是由于β-环糊精将巴氯芬分子中疏水部分包结的结果。
OBJECTIVE: To study the entrapment of baclofen and β-cyclodextrin and the interaction between baclofen and β-cyclodextrin inclusion and bovine serum albumin. Methods: Inclusion behavior of baclofen by β-cyclodextrin was studied by ultraviolet-visible spectrophotometry and nuclear magnetic resonance method. The stoichiometry of inclusion was determined by molar ratio method. Envelopment constants of β-cyclodextrin and fenvalerate were investigated. The interaction between baclofen and inclusion complex on bovine serum albumin (BSA) was studied by fluorescence spectroscopy. Results: The entrapment of baclofen and β-cyclodextrin was spontaneous, and the main driving force was hydrophobic force. Both baclofen and inclusion complex could quench bovine serum albumin statically. CONCLUSION: The entrapment ratio of baclofen to β-cyclodextrin is 1: 1. The main effect of baclofen and bovine serum albumin is hydrophobic, whereas the inclusion and bovine serum albumin are mainly electrostatic. Is due to beta-cyclodextrin baclofen molecule hydrophobic part of the package results.