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目的研究帕金森病患者外周血单核细胞(peripheral blood mononuclear cell,PBMC)脂多糖受体(cluster of differentiation 14,CD14)和toll样受体4(toll-like receptor 4,TLR4)中m RNA的表达并探讨其在疾病发生发展中的临床意义。方法本实验共入组44例帕金森病患者和37例性别、年龄相匹配的健康对照者,并详细记录入组的44例帕金森病患者发病年龄、病程、性别,在关期时对帕金森病患者进行H-Y分期、UPDRSⅡ、UPDRSⅢ、NMSS评分,利用RT-PCR技术检测各研究对象PBMC中CD14和TLR4中m RNA的表达。结果帕金森病患者外周血CD14 m RNA表达水平(1.459±0.658)2~(-△△CT)、TLR4 m RNA(1.408±0.698)2~(-△△CT)表达较健康对照者((1.162±0.631)2~(-△△CT)、(1.122±0.557)2~(-△△CT))均显著增加(P均<0.05)。相关性分析显示帕金森病组CD14 m RNA的表达水平与反应帕金森病疾病严重程度的H-Y分期呈显著正相关(P<0.05)。结论帕金森病患者外周血CD14 m RNA表达与其H-Y分期正相关,提示CD14/TLR4的单核细胞所介导的固有免疫反应可能是帕金森病发生、发展的一个重要因素。
Objective To study the expression of m RNA in cluster of differentiation 14 (CD14) and toll-like receptor 4 (TLR4) in peripheral blood mononuclear cells (PBMCs) of patients with Parkinson’s disease Express and explore its clinical significance in the development of the disease. Methods A total of 44 patients with Parkinson’s disease and 37 healthy controls of the same age and age were enrolled in this study. The age, course and gender of 44 patients with Parkinson’s disease were recorded in detail. HY stage, UPDRS Ⅱ, UPDRS Ⅲ and NMSS score were used to evaluate the expression of m RNA in CD14 and TLR4 in PBMCs of all the subjects. Results The expression of CD14 mRNA (1.459 ± 0.658) 2 ~ (- △△ CT) and TLR4 m RNA (1.408 ± 0.698) 2 ~ (- △△ CT) in peripheral blood of patients with Parkinson ’s disease were significantly higher than those of healthy controls ± 0.631) 2 ~ (- △△ CT), (1.122 ± 0.557) 2 ~ (- △△ CT)) were significantly increased (all P <0.05). Correlation analysis showed that the expression level of CD14mRNA in Parkinson’s disease group was positively correlated with H-Y stage (P <0.05) in response to the severity of Parkinson’s disease. Conclusion The expression of CD14 mRNA in peripheral blood of patients with Parkinson’s disease is positively correlated with the H-Y stage, suggesting that innate immune responses mediated by CD14 / TLR4 monocytes may be an important factor in the development of Parkinson’s disease.