论文部分内容阅读
目的探讨蛇毒心脏毒素（ＣＴＸ）对荷瘤小鼠Ｓ１８０腹水瘤细胞生长抑制的影响。方法小鼠腹腔接种Ｓ１８０活瘤细胞，连续１０ｄ分别腹腔注射ＣＴＸ０．８ｍｇ／ｋｇ、０．４ｍｇ／ｋｇ、０．２ｍｇ／ｋｇ，分析统计荷瘤小鼠的体重变化和癌细胞死亡率，并用有丝分裂完全阻断法分析体内癌细胞的有丝分裂过程。结果ＣＴＸ各剂量组荷瘤小鼠的体重抑制和癌细胞死亡率均有明显的剂量反应关系，与对照组相比有显著的差异（Ｐ＜０．０１）；镜检发现ＣＴＸ能抑制体内癌细胞的有丝分裂，表现在０．４ｍｇ／ｋｇ和０．８ｍｇ／ｋｇ实验组腹水Ｓ１８０细胞处于有丝分裂前期和中期的细胞数量明显减少，其ＭＩ值分别为０．７１％和０．８０％，比对照组显著减少（Ｐ＜０．００１）。结论ＣＴＸ对Ｓ１８０小鼠体内癌细胞的生长有抑制和杀伤作用，并通过干扰和阻断癌细胞的有丝分裂过程，抑制腹水的生长。
Objective To investigate the effect of venom cardiotoxin (CTX) on growth inhibition of S180 ascites tumor in tumor-bearing mice. Methods Mice were inoculated intraperitoneally with S180 live tumor cells, CTX0.8mg / kg, 0.4mg / kg and 0.2mg / kg were intraperitoneally injected for 10 days respectively. The body weight and cancer cell death rate of tumor-bearing mice were analyzed. Complete blockade analysis of mitosis in cancer cells in vivo. Results The dose-response relationship between body weight loss and tumor cell death rate in CTX dose-dependent groups was significant (P <0.01). Microscopy showed that CTX could inhibit in vivo cancer Cell mitosis, the number of ascites S180 cells in the experimental group 0.4mg / kg and 0.8mg / kg pre mitosis and metaphase significantly decreased the number of cells, the MI values were 0.71% and 0.80%, respectively, compared with the control Group was significantly reduced (P <0.001). Conclusion CTX can inhibit and kill the growth of cancer cells in S180 mice and inhibit the growth of ascites by interfering with and blocking the mitosis of cancer cells.