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目的 观察胃肠粘膜保护剂思密达对肝衰竭大鼠内毒素血症的预防作用。方法 SD大鼠65只 ,随机分成3组 ,对照组15只 ,衰竭组25只 ,治疗组25只。衰竭组 :用硫代乙酰胺复制出急性肝衰竭大鼠模型。治疗组 :在肝功能衰竭造模同时应用思密达灌胃 (思密达3g加生理盐水50ml配制 ),每次灌胃2ml,每天2次。对照组 :用生理盐水作对照。3组同时实验 ,第3天剖腹作门静脉内毒素测定及胃肠冲洗液细菌培养 ,并观察胃肠道病理改变。结果 3组大鼠门静脉内毒素水平分别为 :对照组29.33±18.50ng/l;衰竭组105.81±18.82ng/l (较对照组明显升高P<0.01) ;治疗组74.20±21.38ng/l(较衰竭组有明显下降P<0.01)。三组大鼠胃小肠细菌培养结果 :衰竭组较对照组细菌明显增多 (P<0.05) ,治疗组有所下降 ,但无比较意义 (P>0.05) ,胃肠病理改变 :肝功能衰竭组大鼠胃肠扩张明显 ,腔内见大量液体或气体 ,粘膜固有层变薄 ,部分胃粘膜糜烂 ,肝功能衰竭治疗组病变较轻。 结论 由硫代乙酰胺诱导的大鼠急性肝功能衰竭模型门静脉内毒素水平明显增高 ,胃肠道细菌培养提示细菌上移 ,菌落总数明显增多 ,胃肠道存在一定的病变 ,经思密达治疗后 ,门静脉内毒素水平有明显下降 ,细菌总数略有下降 ,胃肠粘膜病变改善
Objective To observe the preventive effect of Smecta, a gastrointestinal mucosal protective agent, on endotoxemia in rats with liver failure. Methods Sixty-five SD rats were randomly divided into three groups, 15 in control group, 25 in failure group and 25 in treatment group. Failure group: thioacetamide replication of acute hepatic failure rat model. Treatment group: Simultaneous application of Smectite intragastric administration in the model of liver failure (Smectite 3g plus 50ml normal saline formulated), each gavage 2ml, 2 times a day. Control group: saline control. 3 groups of simultaneous experiments, the first 3 days of caesarean section for portal vein endotoxin determination and gastrointestinal rinse bacterial culture, and observe the pathological changes of the gastrointestinal tract. Results The levels of portal venous endotoxin in the three groups were 29.33 ± 18.50ng / l in the control group, 105.81 ± 18.82ng / l in the failure group (P <0.01), and 74.20 ± 21.38ng / l in the treatment group Compared with the failure group significantly decreased P <0.01). The result of bacterial culture of stomach intestine in the three groups showed that the number of bacteria in the failure group was significantly higher than that in the control group (P <0.05), but the treatment group was decreased, but no significant difference (P> 0.05). The pathological changes of gastrointestinal tract: Rats gastrointestinal dilatation was obvious, lumen see a lot of liquid or gas, mucosal lamina propria thinning, partial gastric mucosal erosion, liver lesion treatment group lesser lesion. Conclusions The level of portal vein endotoxin in rat model of acute liver failure induced by thioacetamide is obviously increased. The bacterial culture in gastrointestinal tract suggests that the bacteria move upward, the total number of colonies increases obviously, and the gastrointestinal tract has certain pathological changes. After treatment with Smectite After the portal vein endotoxin levels have dropped significantly, the total number of bacteria decreased slightly, gastrointestinal mucosal lesions improved