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目的:研究食管黏膜树突状细胞(DCs)表型特点。方法:内镜下收集健康人食管黏膜标本,Ficoll-Hypaque密度梯度离心法分离食管黏膜标本中的单个核细胞,采用磁珠分选技术分离DCs,流式细胞仪分析食管黏膜DCs表型。结果:健康人食管黏膜含3类单个核细胞:HLA-DRhigh/CD13low,HLA-DRmed/CD13+和HLA-DR-/CD13+;HLA-DRhigh/CD13low细胞表达不同DCs亚群表面标志,是食管黏膜DCs;健康人食管黏膜DCsCD80、CD83、CD86表达水平低,是不成熟DCs。结论:成功分离了食管黏膜DCs,证实HLA-DRhigh/CD13low细胞是食管黏膜DCs。
Objective: To study the phenotypic characteristics of esophageal mucosa dendritic cells (DCs). Methods: The esophageal mucosa specimens of healthy human were collected by endoscopy. The mononuclear cells of esophageal mucosa were isolated by Ficoll-Hypaque density gradient centrifugation. The DCs were isolated by magnetic bead sorting and analyzed by flow cytometry. Results: Three types of mononuclear cells were found in the esophageal mucosa of healthy subjects: HLA-DRhigh / CD13low, HLA-DRmed / CD13 + and HLA-DR- / CD13 + The expression of CD80, CD83 and CD86 in esophageal mucosa of healthy people was low, which was immature DCs. CONCLUSION: Esophageal mucosal DCs were successfully isolated and confirmed that HLA-DRhigh / CD13low cells are esophageal mucosal DCs.