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本文采用反相高效液相色谱(HPLC)法对桃儿七中鬼臼脂素进行分离测定,以甲醇-水(50(?)50)为流动相,UV285nm 检测。在0.243～0.730μg 范围内,峰高值与被测浓度线性良好。回收率为98.83%。共测定了八批样品。操作简便、快速、精密度良好。
In this paper, RP-HPLC method was used for the separation and determination of podophyllotoxin from Taoerqizhong. The methanol-water (50(?)50) was used as the mobile phase and detected at UV285nm. In the range of 0.243 to 0.730 μg, the peak height was well linear to the measured concentration. The recovery rate was 98.83%. A total of eight samples were measured. Easy to operate, fast and accurate.