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作者用双层琼脂培养技术,测定了32例血液学检查正常人的血浆集落刺激活性(CSA)。双层培养体系的上层为1ml.3%的琼脂McCoy!s 5A培养液,内含15%健康产妇胎盘血清和1×10~5个人骨髓有核细胞。底层由1.5ml 0.5%的琼脂营养层组成,内含15%健康产妇胎盘血清和0.1～0.75ml无菌待测血浆,即底层中的待测血浆浓度为6.7%～50%。对照组的底层琼脂中含有0.75ml199培养液。于37℃下培养7～10天后计数不同血浆浓度所刺激产生的集落数(CFUc)。CFUc分为小(<40个细胞),中(40～100个细胞),大(>100个细胞)三种。以血浆浓度为横座标,CFUc数为纵座标,作出剂量-效应曲线。为便于与其他类似实验作比较,可将测得结果作准标化处理,即以最高CFUc数为100%,求出不同浓度血浆所刺激形成的CFUc数为最高数的百分数。测定结果:有69%的血液学正常人可检查出CSA。底层中血浆浓度在从6.7%增加到50%时,所形成的CFUc数及其体积随之增加(血浆为0.1,0.3
The authors measured the colony-stimulating activity (CSA) in 32 hematological normal subjects using double-agar culture. The upper layer of the double-layer culture system is 1 ml of agar McCoy’s 5A liquid containing 15% of healthy maternal placental serum and 1 x 10-5 human bone marrow nucleated cells. The bottom consists of 1.5ml 0.5% agar nutrient layer, containing 15% of healthy maternal placental serum and 0.1 ~ 0.75ml sterile plasma to be tested, that is, the underlying plasma concentration of 6.7% to 50%. The bottom agar in the control group contained 0.75 ml of the199 broth. The number of colonies stimulated by different plasma concentrations (CFUc) was counted after 7 to 10 days of culture at 37 ° C. CFUc is divided into small (<40 cells), medium (40 to 100 cells), large (> 100 cells) three. With plasma concentration as the horizontal axis and CFUc as the vertical axis, a dose-response curve was made. In order to facilitate comparison with other similar experiments, the measured results can be normalized, that is, the maximum number of CFUc formed by different concentrations of plasma stimulated by the highest CFUc number is 100%. Measurement results: 69% of hematological normal people can detect CSA. As the plasma concentration in the bottom layer increased from 6.7% to 50%, the number of CFUc formed and its volume increased (plasma 0.1, 0.3