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目的通过光化学法建立豚鼠耳蜗微循环障碍模型以及观察血管纹、耳蜗毛细胞形态学变化。方法①将豚鼠随机分成5个组。实验组分成3个组,颈外静脉注入四氯四碘荧光素二钠(rose bengal,RB),用波长为(540±40)nm、光强为(500～600)mW/cm2绿光照射打开的听泡,各组选用不同光照时间诱导耳蜗微循环变化。对照组分2个组,对照Ⅰ组仅颈外静脉注入RB而不行光照;对照Ⅱ组不注入RB而仅行光照耳蜗。②分别对5个组动物行听性脑干反应及形态学检测。结果①每个实验组两次ABRⅢ波反应阈差异均有统计学意义(P<0.05);②形态学观察实验组均出现耳蜗微循环障碍的变化,螺旋器以外毛细胞破坏为主。结论采用光化学法可致耳蜗微循环障碍及毛细胞破坏,方法简便,易于实施,重复率高。
Objective To establish a microcirculation disturbance model of guinea pig cochlea by photochemical method and to observe the morphological changes of hair cells and cochlear hair cells. Methods ① Guinea pigs were randomly divided into five groups. The experimental group was divided into three groups. The external jugular vein was infused with rose bengal (RB) and the green light was radiated with (540 ± 40) nm and light intensity (500 ~ 600) mW / cm2 Open the listening bubble, each group choose different lighting time to induce cochlear microcirculation. The control group was divided into two groups. In the control group, RB was injected into the external jugular vein only and no light was applied. In the control group, RB was not injected but only cochlear light. ② Auditory brainstem response and morphological examination were performed on 5 groups of animals respectively. Results ① There were significant differences in ABR Ⅲ wave response thresholds between two groups (P <0.05). ② Morphological changes of cochlear microcirculation were observed in experimental group. Conclusion The photochemical method can cause microcirculation cochlear microcirculation and hair cell destruction, the method is simple, easy to implement, high repetition rate.