论文部分内容阅读
目的建立杏花药材的质量控制方法。方法以绿原酸、芦丁为对照品,建立薄层色谱以鉴别药材。采用紫外波长转换检测的高效液相色谱法同时测定药材中阿魏酸、芦丁及异槲皮苷的含量,色谱柱为YMCPack ODS-A C18柱(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.1%磷酸溶液(125∶105∶770);阿魏酸的检测波长为322nm(0~25 min),芦丁与异槲皮苷的检测波长为255nm(25~70 min);流速:1.0mL/min;柱温:30℃。结果薄层色谱中斑点清晰;阿魏酸、芦丁及异槲皮苷分别在1.452 2~14.521 7μg/mL(r=0.998 9)、27.366 7~273.666 8μg/mL(r=0.997 2)、1.649 9~16.499 0μg/mL(r=0.999 6)范围内线性关系良好,平均加样回收率分别为99.86%、99.70%、100.03%;RSD分别为0.9%、0.8%、1.2%。结论所用方法准确、稳定、可行,可用于杏花药材的质量控制。
Objective To establish the quality control method of apricot herb. Methods chlorogenic acid, rutin as a reference substance, the establishment of thin layer chromatography to identify herbs. The contents of ferulic acid, rutin and isoquercitrin in medicinal materials were determined by HPLC with UV wavelength conversion. The chromatographic column was YMCPack ODS-A C18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of The detection wavelength of ferulic acid was 322 nm (0 ~ 25 min). The detection wavelength of rutin and isoquercitrin was 255 nm (25 ~ 70 min). Flow rate: 1.0 mL / min; Column temperature: 30 ° C. Results The spots in TLC were clear. The contents of ferulic acid, rutin and isoquercitrin were respectively 1.452 2 ~ 14.521 7μg / mL (r = 0.998 9), 27.366 7 ~ 273.666 8μg / mL (r = 0.9972), 1.649 The linearity was within the range of 9-16.499 0 μg / mL (r = 0.999 6). The average recoveries were 99.86%, 99.70% and 100.03%, respectively. The RSDs were 0.9%, 0.8% and 1.2% respectively. Conclusion The method used is accurate, stable and feasible and can be used for quality control of apricot flower.