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目的建立测定速克感冒片中维生素C含量的高效液相色谱(HPLC)法检测方法。方法采用岛津LC-10ATvp型高效液相色谱仪,Diamonsil C18柱(250mm×4.6mm,5μm),流动相为0.02mol/L磷酸二氢钾一乙腈(95:5),检测波长为249nm,流速为1.0mL/min,柱温为25℃。结果维生素C进样量在0.08029～0.3211μg范围内与峰面积线性关系良好((r=0.9998);回收率为99.45%,RSD为0.37%(n=6)。结论方法简便、快速、准确,可用于速克感冒片的维生素C含量测定。
OBJECTIVE To establish a method for the determination of vitamin C in Shukong cold tablets by high performance liquid chromatography (HPLC). Methods A Shimadzu LC-10ATvp high performance liquid chromatograph was used with Diamonsil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.02 mol / L potassium dihydrogen phosphate-acetonitrile (95: 5) The flow rate was 1.0 mL / min and the column temperature was 25 ° C. Results The linear range of peak area was in the range of 0.08029 ~ 0.3211μg (r = 0.9998), the recovery rate was 99.45% and the RSD was 0.37% (n = 6) .Conclusion The method is simple, rapid and accurate, Vitamin C tablets can be used for determination of cold tablets.