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目的 :测定 HIV调节蛋白 Tat在 HIV脑病发生中的作用。方法 :应用逆转录聚合酶链反应 (RT- PCR)方法检测 9例伴有 HIV脑病和 7例不伴有 HIV脑病的爱滋病患者脑组织中 tat、env及 vif m RNA含量。结果 :尽管标本取自患者死后较长时间尸检 ,提取的脑组织 RNA已有明显降解 ,但在伴有 HIV脑病病例中 ,tat m RNA和env m RNA检出率分别为 4 / 9和 5 / 9,而在 7例非伴有 HIV脑病的病例中 ,二项指标均为零 ;vif m RNA在两组病例的检出率分别为 5 / 9和 2 / 7。结论 :Tat对 HIV脑病的神经病理改变起着重要作用。
Objective: To determine the role of HIV regulatory protein Tat in the development of HIV encephalopathy. Methods: The contents of tat, env and vif m RNA in brain tissue of 9 AIDS patients with HIV and 7 AIDS patients without HIV encephalopathy were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Although the specimens were taken from the autopsy of the patient after a long period of death, the RNA extracted from the brain tissue had been significantly degraded. However, the detection rates of tat m RNA and env m RNA in cases with HIV encephalitis were 4/9 and 5, respectively / 9, while in seven cases of non-HIV encephalopathy cases, the two indicators were zero; vif m RNA in the two groups of cases were detected in 5/9 and 2/7. Conclusion: Tat plays an important role in the neuropathological changes of HIV encephalopathy.