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目的:建立地肤子的高效液相色谱(HPLC)指纹图谱。方法:选择安捷伦TC-C_(18)色谱柱,二极管阵列检测器(DAD),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,总流速1 m L/min,检测波长355 nm,柱温25℃,进样量为10μL。采用《中药色谱指纹图谱相似度评价系统A版》对10批次的地肤子进行相似度分析,选择共有峰的相对峰面积对样品进行聚类分析。结果:HPLC指纹图谱共发现19个共有峰,其中14号峰为芦丁。10批次地肤子与对照图谱相似度均>0.95。聚类分析结果显示,地肤子药材可归为3类,相同产地药材可归为1类,说明不同产地药材中成分的含量有明显区别。结论:该方法稳定性、重复性良好,可用于地肤子药材的质量评价和鉴定。
Objective: To establish a HPLC fingerprinting of Radix Methods: Agilent TC-C 18 column and diode array detector (DAD) were used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution with a gradient of 1 m L / min. The detection wavelength was set at 355 nm. 25 ℃, injection volume of 10μL. The similarity analysis of 10 batches of Kadsura kaempferu was carried out using the “Chinese medicine chromatographic fingerprint similarity evaluation system version A”, and the relative peak area of the common peaks was selected to cluster the samples. RESULTS: A total of 19 common peaks were identified by HPLC fingerprinting. The 14th peak was rutin. 10 batches of kudzu and control maps similarity> 0.95. Cluster analysis showed that the Kadsura herbs can be grouped into three categories, the same origin of medicinal herbs can be classified as a category, indicating that the ingredients of different origin medicinal ingredients were significantly different. Conclusion: The method is stable, reproducible and can be used for the quality evaluation and identification of Radix.