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目的 :探讨粒细胞集落刺激因子 (G -CSF)对大鼠缺血性脑梗死的治疗作用。方法 :采用线栓法制作大鼠缺血性脑梗死模型 ,1h后腹腔注射G -CSF 6 0 μg/kg。TTC、HE染色及免疫组化检测脑梗死灶体积、病理改变及CD34阳性细胞浸润情况。结果 :G -CSF治疗组大鼠脑梗死灶体积明显小于对照组 (P <0 0 1) ;其病理损伤程度较对照组轻 ;脑梗死部位出现CD34阳性单个核细胞浸润 ,并有CD34阳性呈锥状并且带有突起的神经样细胞生长 ,对照组未发现CD34阳性细胞。结论 :G -CSF可减轻大鼠缺血性脑梗死程度 ,减少脑梗死体积 ,其机理可能是G -CSF对缺血神经元具有保护作用 ,并动员自体骨髓干细胞 ,促进脑组织的再生与修复。
Objective: To investigate the therapeutic effect of granulocyte-colony stimulating factor (G-CSF) on ischemic cerebral infarction in rats. Methods: The rat model of ischemic cerebral infarction was established by thread occlusion method. After 1h, G-CSF was injected intraperitoneally (60 μg / kg). TTC, HE staining and immunohistochemical detection of cerebral infarction volume, pathological changes and CD34-positive cell infiltration. Results: The volume of cerebral infarction in G-CSF group was significantly smaller than that in control group (P <0.01). The pathological changes of G-CSF group were lighter than those in control group. CD34-positive mononuclear cells infiltrated in cerebral infarction and CD34 positive Conical and neurite-like cells with protrusions grew, while no CD34-positive cells were found in the control group. CONCLUSION: G-CSF can reduce the degree of ischemic cerebral infarction and decrease the volume of cerebral infarction in rats. The mechanism may be that G-CSF can protect ischemic neurons and mobilize autologous bone marrow stem cells and promote the regeneration and repair of brain tissue .