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[目的]研究消痰利水酊剂对艾氏腹水瘤(EAC)小鼠的作用及其可能的机制。[方法]昆明种实验小鼠腹腔内注射0.2 ml EAC E2G8细胞株悬液(瘤细胞浓度4×106/ml)制备腹水瘤小鼠模型。成模小鼠分为荷瘤(对照)组、消痰利水酊剂(酊剂)组、环磷酰胺(CTX)组、酊剂加CTX(联用)组,分别给予荷瘤对照、腹壁外用消痰利水酊剂、腹腔注射CTX、腹壁外用酊剂加腹腔注射CTX联用。观察各组小鼠体重、死亡后排净腹水之净体重、死亡时腹水重量、生存时间,ELISA法检测各组腹水上清液血管内皮生长因子(VEGF)、肝素酶(HPA)水平。[结果]酊剂组可降低荷瘤小鼠种瘤后0~12 d体重恶化趋势(P<0.05),与CTX腹腔注射联用可加强此作用(P<0.05),并能减少小鼠死亡时腹水量(P<0.05),有效提高荷瘤小鼠死亡时净体重(P<0.05),效果优于单纯予以CTX(P<0.05)。酊剂组、CTX组、联用组均能明显降低小鼠腹水上清液中HPA水平(P<0.05)。但各组生存时间差异无统计学意义(P>0.05)。[结论]消痰利水酊剂与CTX联用更可加强延缓艾氏腹水瘤小鼠体重变化,抑制恶性腹水的生成,增加小鼠净体重,改善小鼠生存质量,减少化疗部分不良反应。其作用机制可能与下调腹水中HPA的水平从而降低腹膜及血管通透性相关。
[Objective] To investigate the effect of Xiaotan Lishui tincture on Ehrlich ascites tumor (EAC) mice and its possible mechanism. [Method] Kunming experimental mice were injected intraperitoneally with 0.2 ml EAC E2G8 cell suspension (tumor cell concentration 4 × 106 / ml) to prepare mouse model of ascites tumor. The model mice were divided into three groups: tumor-bearing (control group), Xiaotan Lishui tincture (tincture) group, cyclophosphamide (CTX) group, tincture plus CTX Tincture, intraperitoneal injection of CTX, abdominal topical tincture plus intraperitoneal injection CTX combined. The body weight, the net weight of ascites after death, the weight of ascites at the time of death and the survival time were observed. The levels of vascular endothelial growth factor (VEGF) and heparanase (HPA) in ascites supernatant of each group were measured by ELISA. [Result] The tincture group could reduce the weight loss tendency of tumor-bearing mice from 0 to 12 d after tumor implantation (P <0.05). Combined with intraperitoneal injection of CTX, the tincture group could enhance this effect (P <0.05) and reduce the death of mice Ascites volume (P <0.05), effectively increasing the body weight of tumor-bearing mice at the time of death (P <0.05), was better than that of CTX alone (P <0.05). The tincture group, CTX group and combination group could obviously reduce the level of HPA in the mouse ascites supernatant (P <0.05). However, there was no significant difference in survival time between groups (P> 0.05). [Conclusion] Xiaotan Lishui tincture combined with CTX can further delay the body weight change of Ehrlich ascites tumor, inhibit the formation of malignant ascites, increase the net body weight of mice, improve the quality of life of mice and reduce the adverse reaction of chemotherapy. Its mechanism of action may be related to down-regulating the level of HPA in ascites and thus reducing peritoneal and vascular permeability.